敬告作者朋友
最近我们发现,有一些假冒本刊在线投稿系统的网站,采用与《麦类作物学报》相似的网页、网址和邮箱发送征稿通知以及收取审稿费、版面费的信息,以骗取钱财。详细情况见【通知公告】栏的“再次提醒作者朋友:谨防上当受骗!!!”

关闭
高温胁迫对小麦花药活性氧代谢的影响
Effect of High Temperature Stress on Reactive Oxygen Metabolism of Wheat Anther
投稿时间:2019-10-19  修订日期:2019-11-21
DOI:
中文关键词:  小麦  花药  高温胁迫  活性氧代谢  抗氧化酶  
英文关键词:Wheat  Anther  High temperature stress  Reactive oxygen metabolism  Antioxidant enzymes
基金项目:
作者单位E-mail
卢奕霏 长江大学农学院/主要粮食作物产业化湖北省协同创新中心 425990666@qq.com 
顾迎港   
陈威   
王娜   
康珍   
侯泽豪   
张迎新   
方正武   
马东方   
刘易科   
朱展望   
张改生   
王书平 长江大学农学院/主要粮食作物产业化湖北省协同创新中心 wangshuping2003@126.com 
摘要点击次数: 51
全文下载次数: 0
中文摘要:
      为为研究小麦花药在高温胁迫下的氧化应答机制,本研究以高温胁迫后(42℃± 1℃)不同发育时期的花药(单核后期、二核期和三核期)为供试材料,比较分析了小麦花药中超氧阴离子自由基(O2-·)的生成速率、过氧化氢(H2O2)和丙二醛(MDA)的含量以及主要抗氧化酶活性变化。结果表明,在高温胁迫后,小麦花药的整个发育时期O2-·生成速率、H2O2和MDA的含量均显著高于对照组,其中O2-·生成速率呈现出由“低–高”平衡上升的趋势,在单核后期高温胁迫与对照之间的差异则高于其他花药发育时期;而高温胁迫后的H2O2和MDA含量在二核期与对照组呈现较大差异。同时,高温胁迫花药中SOD活性、POD活性、CAT活性均显著低于对照。由此可知,高温胁迫打破了小麦花药内活性氧的产生与抗氧化体系代谢之间的平衡,并随着花药的发育而不断加剧,使得在发育关键期的小麦花药长期处于严重的氧胁迫环境中而造成败育。研究结果为进一步阐明小麦花药对高温胁迫的响应机制奠定了基础,为认识高温胁迫下小麦花药败育的机理提供了证据。
英文摘要:
      Abstract: In order to reveal the mechanism of oxidative response of wheat anthers under high temperature stress, wheat anthers of high-temperature stress (42℃± 1℃) at different developmental stages (later-uninucleate stage, binucleate stage, and trinucleate stage) were used to study the rate of superoxide anion (O2-·), the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA), and the activities of major antioxidant enzymes. The results showed that after high-temperature stress, the O2-· production rate, H2O2 and MDA content of wheat anther were significantly higher than those in the control, and the O2-·production rate showed a trend of "low-high", and the difference between the high-temperature stress and control at the later-uninucleate stage was higher than that in other development stages. However the H2O2 and MDA contents in the high-temperature stress and the control was significantly different at the binucleate stage. Meanwhile, the activities of SOD, POD and CAT in high temperature stress were significantly lower than those in the control. Accordingly, high-temperature stress breaks the balance between the production of reactive oxygen species and the metabolism of antioxidant system in wheat anther, and it was intensified with the development of anther, which makes the wheat anther at the critical period of development in a serious oxygen stress environment for a long time and causes abortion. The results lay a foundation for further elucidating the response mechanism of wheat anther to high temperature stress, and also provide evidence for understanding the mechanism of wheat anther abortion under high temperature stress.
  查看/发表评论  下载PDF阅读器
关闭

您是第9691619位访问者
版权所有麦类作物学报编辑部
京ICP备09084417号
技术支持: 本系统由北京勤云科技发展有限公司设计