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小麦纹枯病抗性QTL的分析
Analysis of QTLs for the resistance to sharp eyespot in wheat
投稿时间:2019-09-30  修订日期:2019-11-29
DOI:
中文关键词:  小麦  纹枯病  抗性  QTL
英文关键词:Wheat  Wheat sharp eyespot  resistance  QTL
基金项目:转基因生物新品种培育重大专项
作者单位E-mail
周淼平 江苏省农业科学院粮食作物研究所/江苏省农业生物学重点实验室 mpzhou2000@163.com 
姚金保   
杨学明   
张鹏   
余桂红   
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中文摘要:
      小麦纹枯病是世界性的小麦重要病害,培育和使用抗病品种是减轻纹枯病危害最经济和有效的手段。为了挖掘更多小麦纹枯病抗性QTL用于小麦标记辅助育种,本研究构建了CI12633和扬麦158重组自交系群体;采用二代测序方法开发SNP分子标记,并对群体各家系进行基因型分析,构建遗传连锁图;结合牙签接种和病麦粒接种的纹枯病抗性鉴定资料,对小麦纹枯病抗性QTL进行定位。结果显示,构建的遗传连锁图包含分子标记3355个,遗传距离2510.7cM,共有31个连锁群,均能分配到相应的染色体;在5A(2)、6A、1B、2B、3B、4B、5B、6B(2)、7B、1D、2D(2)、4D和7D染色体共发现16个与小麦纹枯病抗性相关的QTL,单个QTL可解释9.0%-26.8%的表型效应;除了7B染色体的QTL由感病品种扬麦158贡献外,其余QTL均来自抗病品种CI12633;3B、7D和5A(Chr5A_564101963)染色体的QTL与已有报道一致,其余均为新发现QTL。发现的QTL和紧密连锁分子标记将为今后的标记辅助育种以及抗性基因的克隆提供帮助。
英文摘要:
      Wheat sharp eyespot (WSE) is an important disease worldwide. It is an economic and effective measure to develop and apply the cultivars with disease resistance. In order to exploit more QTLs resistant to WSE for marker- assisted breeding, the recombinant inbred lines (RILs) were constructed by CI12633 and Yangmai 158 crossing. Molecular markers such as SNP were developed by second-generation sequencing method, and the genotypes of RILs were analyzed to construct genetic linkage map. Combined with the resistance evaluation data of WSE by toothpick inoculation and disease grain inoculation, the QTLs of resistance to WSE was mapped in the chromosome. The results showed that there were 31 linkage groups in the genetic linkage map, containing 3355 molecular markers with a genetic distance of 2510.7cM, all of which could be allocated to the corresponding chromosomes based on SSR markers. In chromosome 5A (2), 6A, 1B, 2B, 3B, 4B, 5B, 6B (2), 7B, 1D, 2D (2), 4D and 7D, 16 QTLS were found for WSE resistance. Except the QTL of 7B chromosome was contributed by Yangmai 158, the other QTLs were all from the resistant variety of CI12633. The QTLs of chromosomes 3B, 7D and 5A (Chr5A_564101963) were consistent with those reported, while the rest were newly discovered QTLs. The QTLs developed and its molecular markers linked tightly will be helpful for marker-assisted breeding and cloning of resistance genes in the future.
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