| Wheat sharp eyespot (WSE) is an important disease worldwide. It is an economic and effective measure to develop and apply the cultivars with disease resistance. In order to exploit more QTLs resistant to WSE for marker- assisted breeding, the recombinant inbred lines (RILs) were constructed by CI12633 and Yangmai 158 crossing. Molecular markers such as SNP were developed by second-generation sequencing method, and the genotypes of RILs were analyzed to construct genetic linkage map. Combined with the resistance evaluation data of WSE by toothpick inoculation and disease grain inoculation, the QTLs of resistance to WSE was mapped in the chromosome. The results showed that there were 31 linkage groups in the genetic linkage map, containing 3355 molecular markers with a genetic distance of 2510.7cM, all of which could be allocated to the corresponding chromosomes based on SSR markers. In chromosome 5A (2), 6A, 1B, 2B, 3B, 4B, 5B, 6B (2), 7B, 1D, 2D (2), 4D and 7D, 16 QTLS were found for WSE resistance. Except the QTL of 7B chromosome was contributed by Yangmai 158, the other QTLs were all from the resistant variety of CI12633. The QTLs of chromosomes 3B, 7D and 5A (Chr5A_564101963) were consistent with those reported, while the rest were newly discovered QTLs. The QTLs developed and its molecular markers linked tightly will be helpful for marker-assisted breeding and cloning of resistance genes in the future.