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大麦EMS突变体库构建和突变体筛选
Construction of EMS-treated Mutant Library and Mutant Phenotypic Analysis in Barley
投稿时间:2019-08-04  修订日期:2019-10-13
DOI:
中文关键词:  大麦  EMS诱变  突变体
英文关键词:Barley  EMS inducement  Mutant
基金项目:国家大麦青稞产业技术体系(CARS-05), 江苏省农业自主创新[CX(17)3056], 江苏农业科技援藏-青稞种质资源鉴定及新品系选育[JATS(2018)139], 上海市科技兴农项目(2019-02-08-00-08-F01109)
作者单位E-mail
栾海业 江苏沿海地区农业科学研究所 luanhaiye@163.com 
沈会权 江苏沿海地区农业科学研究所 jsycshq@163.com 
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中文摘要:
      为给大麦重要功能基因研究以及大麦遗传育种提供新的种质资源,通过甲基磺酰乙酯(EMS)诱变处理优质、高抗黄花叶病大麦品种苏啤6号,从2400个M2代单株中筛选出152个突变株,突变频率为6.33%。其中,幼苗习性突变单株36个,突变率为1.50%;叶部突变单株34个,突变率为1.42%;茎部突变单株33个,突变体率1.38%;穗部突变单株22个,突变率为0.92%;成熟期和育性突变单株27 个,突变率为1.13%。经过M3代验证,获得可稳定遗传的各类突变株系43个。
英文摘要:
      In order to provide a basic material for the study of the functional genomics and genetic breeding of barley. Supi 6, a high quality malting and resistant to yellow mosaic disease barley was treated with the chemical mutagen ethyl methane sulfonate (EMS). Of the 2400 M2 generation plants screened, a total of 152 had significantly visible mutant phenotypes with a mutation frequency of 6.33%. Of these, 36 plants were seedling habits mutants, with a mutation frequency of 1.50%; 34 plants were leaf mutants, with a mutation frequency of 1.42%; 33 plants were stalk mutants, with a mutation frequency of 1.38%; 22 plants were spike mutants, with a mutation frequency of 0.92%; 27 plants were mature period and fertility mutants, with a mutation frequency of 1.13%. In total, 43 stably inherited lines were obtained by detailed agronomic trait investigation at M3 generation.
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