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4份圆锥小麦-乌拉尔图小麦双二倍体的分子细胞遗传学鉴定
Molecular cytogenetic identification of four new synthetic amphiploids from T. turgidum ssp. turgidum and T. urartu
投稿时间:2019-07-09  修订日期:2020-01-07
DOI:
中文关键词:  乌拉尔图小麦  圆锥小麦  双二倍体  荧光原位杂交  寡核苷酸探针
英文关键词:T. urartu  T. turgidum ssp. turgidum  amphiploids  FISH  Oligo probe
基金项目:国家重点研发计划项目(2017YFD0100904)、国家自然科学(31671682)和四川省教育厅项目(16CZ0004)
作者单位E-mail
李洪雨 四川农业大学小麦研究所 846840630@qq.com 
刘小娟   
梁东玉   
刘登才   
郝明   
袁中伟   
甯顺腙   
张连全 四川农业大学小麦研究所 zhanglianquan1977@126.com 
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中文摘要:
      为了向普通小麦转移乌拉尔图小麦(Triticum urartu Thum. ex Gandil., AuAu, 2n=2x=14)的优异基因,2014-2015年度我们利用3个中国的四倍体小麦地方品种圆锥小麦(T. turgidum L. ssp. turgidum, AABB, 2n=4x=28)与引进的4个乌拉尔图小麦杂交,对获得的杂种F1植株进行0.1%秋水仙素溶液加倍处理,成功得到了4份圆锥小麦-乌拉尔图小麦双二倍体(AABBAuAu)。本研究对这4份双二倍体的农艺性状、染色体组成、及高分子量谷蛋白亚基组成进行了鉴定。农艺性状调查表明,4份双二倍体的株高介于108.45~139.43 cm,分蘖数介于7.3~17.5个,穗长介于10.23~12.17 cm,小穗数介于16.26~22.06个,自交结实率介于37.77%~70.46%。成株期条锈病抗性调查表明,4份双二倍体对目前的流行条锈菌混合生理小种(条中31、条中32、条中33、条中34和水源11-4)均表现为高抗。荧光原位杂交鉴定表明,利用5对寡核苷酸序列探针Oligo-pSc119.2-1、Oligo-pTa535-1、Oligo-pTa71-2、 pTa-713和(AAC)5进行FISH分析,区分出双二倍体的染色体的归属。花粉母细胞染色体配对观察表明,这些双二倍体减数分裂中期I,染色体大多配对成二价体,仅有少量的单价体、三价体和四价体。SDS-PAGE分析表明,2份亲本乌拉尔图小麦的1Ay亚基在对应的双二倍体中得到了表达。这些双二倍体可作为新的资源材料用于普通小麦的遗传改良。
英文摘要:
      In order to transfer the useful gene of T. urartu to common wheat, four synthetic T. turgidum ssp. turgidum - T. urartu amphiploids (AABBAuAu) were produced by the colchicine treatment of the F1 hybrids between the Chinese tetraploid landraces of T. turgidum ssp. turgidum and T. urartu in 2014-2015. In this study, the agronomic character, chromosome constitutions and high molecular weight subunits of these amphiploids were investigated. Investigation of agronomic characters of these amphiploids showed that the plant height was ranged from 108.45 cm to 139.43 cm, the tiller number ranged from 7.3 to 7.5, ear length ranged from 10.23 cm to 12.17 cm, the spikelets ranged from 16.26 to 22.06, the selfed seedset ratios ranged from 37.77% to 70.46%. Investigation of resistance to stripe rust at adult stage suggested that all four amphiploids exhibited high resistance to predominant stripe rust races CYR31, CYR32, CYR33, CYR34, and SY11-4. Individual chromosomes of the T. turgidum ssp. turgidum - T. urartu amphiploids could be identified by multicolor fluorescence in situ hybridization using the combination of oligonucleotides probes of Oligo-pSc119.2-1, Oligo-pTa535-1, Oligo-pTa71-2, pTa-713 and (AAC)5. The 42 chromosomes of the amphiploids usually paired with bivalents, along with a small number of univalent, trivalent and tetravalent. SDS-PAGE analysis showed that 1Ay subunits from two different T. urartu parents were expressed in the corresponding amphiploids. These T. turgidum ssp. turgidum - T. urartu amphiploids are new resources for bread wheat improvement
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