| 王宇凡，夏英杰，时 佳，陈咸宁，黄 林，张跃强，伍碧华.小麦TaTPK基因的抗条锈病功能分析[J].麦类作物学报,2026,(7):871 |
| 小麦TaTPK基因的抗条锈病功能分析 |
| Functional Analysis of TaTPK in Wheat Resistance to Stripe Rust |
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| DOI： |
| 中文关键词: 小麦 条锈病 蛋白激酶 病毒诱导的基因沉默 |
| 英文关键词:Wheat Stripe rust Protein kinase VIGS |
| 基金项目:新疆维吾尔自治区农业科学院稳定支持项目（xjnkywdzc-2024001-01） |
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| 中文摘要: |
| 为解析编码Pti1-like酪氨酸蛋白激酶的基因TaTPK对条形柄锈菌（Puccinia striiformis West f. sp. tritici）的抗性机制，以小麦抗病品种蜀麦126和感病品种台长29为材料，通过qRT-PCR、亚细胞定位、病毒诱导的基因沉默和组织化学观察等技术，对TaTPK的功能进行了分析。结果表明，在条锈菌CYR34侵染后，抗病品种蜀麦126中TaTPK的表达量显著高于对照及感病品种台长29。TaTPK编码1个膜蛋白。利用基因沉默技术沉默TaTPK后，蜀麦126对条锈菌小种CYR34的抗性减弱，表现为孢子堆增多，菌丝生长加快，且侵染点附近H2O2积累减少，孢子覆盖叶面积百分比增加。综上，TaTPK通过调控活性氧积累参与小麦对条锈病的抗性反应，是小麦抗病育种的候选基因资源。 |
| 英文摘要: |
| To elucidate the molecular mechanism of the wheat gene TaTPK, which encodes a Pti1-like tyrosine protein kinase, in resistance to stripe rust(Puccinia striiformis West f. sp. tritici), this study used a disease-resistant wheat variety Shumai 126 and a susceptible variety Taichang 29. The function of TaTPK was analyzed using techniques such as qRT-PCR, subcellular localization, virus-induced gene silencing, and histochemical observation. The results showed that after CYR34 infection, the expression level of TaTPK in the resistant variety Shumai 126 was significantly higher than in the susceptible variety Taichang 29. TaTPK encodes a membrane protein, and silencing TaTPK through gene silencing technology weakened the resistance of Shumai 126 to the stripe rust race CYR34, manifested by an increased number of spore pustules, accelerated hyphal growth, decreased H2O2 accumulation near infection sites, and a higher percentage of leaf area covered by spores. In summary, TaTPK participates in the wheat resistance response to stripe rust by regulating reactive oxygen accumulation. This study provides a candidate gene resource for durable wheat disease resistance breeding. |
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