In order to explore the response of nitrogen accumulation and transport and metabolism enzyme activity in top-three leaves,grain yield and processing quality of strong gluten wheat to irrigation amount,two strong gluten wheat varieties(Zhongmai 998 and Jinnong 7) were selected to analyze the effects of irrigation amount[no irrigation in spring(W0); jointing water and flowering water in spring,250 m3·hm-2 each time(W25); Jointing water and flowering water in spring,500 m3·hm-2 each time(W50)] on nitrogen metabolism enzyme activity,nitrogen content and nitrogen accumulation and transport in top-three leaves,grain protein content,grain yield,water use efficiency and grain processing quality of strong gluten wheat. The results showed that in the same period,the activities of NR and GS,nitrogen transport and contribution rate of pre-anthesis nitrogen to grain ranked as flag leaf > second leaves > third leaves. With the increase of irrigation amount,the NR and GS activities of grains,flag leaves and the second leaves were improved,but there was no significant effect on the NR activity in the third leaves. The nitrogen content at flowering stage and pre-anthesis nitrogen transport of flag leaf under W25 treatment were significantly higher than those of the other two treatments,but was the highest in the second and third leaves under the treatment of W50. With the increase of irrigation amount,protein yield first increased and then decreased; grain yield increased,but water use efficiency decreased significantly. The gluten index,water absorption,development time,stability time,tensile resistance,extensibility and tensile area of W25 treatment were the best or the second. To sum up,when irrigated at jointing and flowering stages in spring,250 m3·hm-2 each time,it is most conducive to the coordinated improvement of protein content,yield,and water use efficiency of strong gluten wheat,and thus the grain processing quality can be improved. |