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李鸽子,刘 金,张丹丹,肖向红,王永华,郭天财,康国章.TaHIPP32 TaHAK1表达及小麦缺钾胁迫耐性的关系[J].麦类作物学报,2023,(2):131
TaHIPP32 TaHAK1表达及小麦缺钾胁迫耐性的关系
TaHIPP32 to Potassium Stress Tolerance and the Expression Levels of TaHAK1 in Wheat
  
DOI:10.7606/j.issn.1009-1041.2023.02.01
中文关键词:  小麦  缺钾胁迫  钾转运蛋白   TaHIPP32基因  病毒诱导基因沉默
英文关键词:Wheat  Potassium deficiency stress  Potassium transporter   TaHIPP32  BSMV-VIGS
基金项目:河南省自然科学基金-优秀青年基金项目(212300410045)
作者单位
李鸽子,刘 金,张丹丹,肖向红,王永华,郭天财,康国章 1.河南农业大学农学院/国家小麦工程技术研究中心河南郑州 450046 2.省部共建小麦玉米作物学 国家重点实验室河南郑州 450046 3.河南省作物生长发育调控重点实验室河南郑州 450046 
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中文摘要:
      钾是植物生长发育必需的三大营养元素之一。高亲和性钾转运蛋白(HAK)能加强植物在缺钾胁迫时钾的吸收和转运。本课题组前期研究发现,小麦 TaHAK1基因在缺钾胁迫条件下显著上调表达,并筛选得到可能调控 TaHAK1基因表达的上游转录因子基因 TaHIPP32,但这两个基因在缺钾胁迫中的作用机制尚不清楚。本研究首先对 TaHIPP32基因进行了系统发育和亚细胞定位分析,然后利用双荧光素酶验证 TaHIPP32 TaHAK1基因的互作,并以河南省大面积推广的小麦品种百农207为试验材料,利用大麦条纹花叶病毒诱导基因沉默(BSMV-VIGS)技术分析缺钾胁迫处理下 TaHIPP32基因沉默对小麦植株的影响。系统发育和亚细胞定位分析表明, TaHIPP32基因与水稻 OsHIPP33基因的亲缘关系最近,且其编码的蛋白定位于细胞膜上。双荧光素酶试验表明,TaHIPP32蛋白能够与 TaHAK1基因启动子互作。通过BSMV-VIGS技术瞬时沉默 TaHIPP32基因,发现该基因沉默后,小麦植株对缺钾胁迫更为敏感,植株生长受到抑制,干重显著降低,丙二醛(MDA)含量显著提高;缺钾胁迫处理下,沉默 TaHIPP32基因的植株中, TaHAK1 TaHIPP32基因的表达均被抑制,且植株体内钾元素从地下部到地上部的转移系数显著降低,说明 TaHIPP32基因通过调节 TaHAK1基因的表达来影响植株体内钾元素的转运。
英文摘要:
      Potassium is one of the three essential nutrients for plant growth and development.High affinity potassium transporter(HAK) can enhance potassium uptake and transport in plants under low potassium stress. Our previous studies found that TaHAK1 was significantly induced under potassium deficiency(DK) stress,and the upstream transcription factor gene TaHIPP32 may regulate the expression of TaHAK1.However,the mechanism of these two genes under DK stress remains unclear. In this study, TaHIPP32 was analyzed by phylogenetic tree and subcellular localization,and the interaction between TaHIPP32 and TaHAK1 was verified by dual-luciferase. The wheat cultivar Bainong 207 was used as the test material,and the effect of TaHIPP32 gene silencing on wheat under potassium deficiency stress were analyzed by using the barley stripe mosaic virus-virus induced gene-silencing(BSMV-VIGS) method. Phylogenetic and subcellular localization analysis indicated that TaHIPP32 was closely related to rice OsHIPP33,and TaHIPP32 is located in cell membrane.Dual-luciferase analysis showed that TaHIPP32 interacted with the promotor of TaHAK1. Transient silencing of TaHIPP32 by BSMV-VIGS method showed that wheat plants were more sensitive to DK stress,which exhibited inhibited growth,decreased dry weight but increased malondialdehyde(MDA) content after TaHIPP32 gene silencing. In TaHIPP32 gene silenced plants,the expression of TaHAK1 and TaHIPP32 were inhibited by DK stress,and the transfer coefficient of potassium in plants from underground to aboveground was significantly decreased,suggesting that TaHIPP32 regulated the expression of TaHAK1 to affect the transport of potassium in plants.
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