| 齐 越，于 晶，苍 晶，田 宇，彭瞰看，王多佳，王政委，任治鹏，梁 伊，王晓楠，孟 婧.小麦DEAD-box基因家族鉴定及其低温下的表达模式分析[J].麦类作物学报,2022,(2):127 |
| 小麦DEAD-box基因家族鉴定及其低温下的表达模式分析 |
| Identification of DEAD-box Gene Family and Expression Analysis in Response to Cold Stress in Wheat |
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| DOI：10.7606/j.issn.1009-1041.2022.02.01 |
| 中文关键词: 小麦 DEAD-box 生物信息学 qRT-PCR 低温胁迫 |
| 英文关键词:Wheat DEAD-box Bioinformatics analysis qRT-PCR Cold Stress |
| 基金项目:国家自然科学基金项目（31971831） |
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| 中文摘要: |
| DEAD-box RNA解旋酶是RNA代谢途径中的关键酶，在RNA代谢过程中调控植株的生长发育和抗逆性。目前，尚未见有人对小麦DEAD-box基因家族进行系统鉴定与分析。为探究小麦DEAD-box基因的功能，本研究以水稻DEAD-box基因家族基因为参照，从小麦全基因组数据库中共鉴定到134个小麦DEAD-box家族基因，并利用生物信息学对其家族成员的理化性质、基因结构、进化树和共线性进行分析。结果表明，小麦DEAD-box蛋白多数为弱碱性蛋白，亚细胞定位分布广泛，核分布最多；小麦与水稻DEAD-box家族基因亲缘性较高，小麦DEAD-box蛋白结构域与核心基序排列有序稳定，高度保守；小麦DEAD-box家族基因在进化过程中可能发生了节段性复制事件，水稻与小麦DEAD-box基因无序共线性连线，说明DEAD-box基因在进化过程中可能存在染色体异位突变。进一步以强抗寒冬小麦品种东农冬麦1号(Dn1)和东农冬麦2号(Dn2)为材料，对其转录组库中低温差异表达的7个DEAD-box基因进行qRT-PCR分析，结果表明，这7个基因在不同品种和不同组织中表达量均有差异。Dn1中DEAD-box基因的表达量在 -10 ℃达到峰值，而Dn2中DEAD-box基因的表达量在-25 ℃达到峰值。 |
| 英文摘要: |
| DEAD-box RNA helicase is a key enzyme in RNA metabolism pathway,which regulates plant growth and development and stress resistance during RNA metabolism.At present,there is no systematic identification and analysis of the DEAD-box gene family in wheat.In order to explore the function of the DEAD-box gene in wheat,using the rice DEAD-box gene family as the reference,134 DEAD-box genes were identified from the wheat genome database.The physicochemical properties,gene and protein structure,phylogenetic tree and collinearity of wheat DEAD-box gene family members were analyzed by bioinformatics.The results showed that the subcellular localization of DEAD-box protein in wheat was widespread,among which most were distributed in the nuclear.The relationship between wheat and rice DEAD-box family is closer than that of Arabidopsis thaliana.The structure domain and core motifs of wheat DEAD-box protein are orderly and stable,and highly conserved in evolution.Segment replication events may occur in the evolution of wheat DEAD-box genes,and some disordered collinear lines between rice and wheat suggest that there may be chromosomal ectopic mutations in the evolution of the DEAD-box genes.Using strong cold-resistant winter wheat varieties Dongnongdongmai 1 (Dn1) and Dongnongdongmai 2 (Dn2) as materials,qRT-PCR analysis of seven DEAD-box genes which differentially expressed in the transcriptome library at low temperature was performed.The results showed that the expression levels of the seven DEAD-box genes in different tissues and wheat varieties were different.The DEAD-box genes expression in Dn1 mostly peaked at -10 ℃,while the DEAD-box gene expression in Dn2 mostly peaked at -25 ℃. |
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