| 戴 双，郭 军，程敦公，曹新有，巨 伟，訾 妍，吉万全，宋健民.叶片衰老抑制基因 PSAG12-IPT在小麦遗传改良中的应用[J].麦类作物学报,2020,(11):1290 |
| 叶片衰老抑制基因 PSAG12-IPT在小麦遗传改良中的应用 |
| Utilization of Leaf Senescence-Inhibition Gene PSAG12-IPT for Wheat Improvement |
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| DOI：10.7606/j.issn.1009-1041.2020.11.02 |
| 中文关键词: 小麦 叶片衰老 PSAG12-IPT基因 叶绿素含量 |
| 英文关键词:Wheat Leaf senescence PSAG12-IPT gene Chlorophyll content |
| 基金项目:转基因生物新品种重大专项（2016ZX08002003) |
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| 中文摘要: |
| 为了解叶片衰老抑制基因 PSAG12-IPT在小麦遗传改良中的作用，利用共表达PSAG12和IPT的转基因小麦材料（TIPT-XN1376）与推广小麦品种陕麦159、周麦18、远丰175、西农979和郑麦9023杂交，并对其后代进行分子标记辅助选择和叶绿素含量测定，研究 PSAG12-IPT基因在小麦中的遗传特点及其与衰老和叶片叶绿素含量的关系。结果表明，150个转基因株系中，携带 PSAG12-IPT植株与不携带 PSAG12-IPT植株的分离比为110∶40，χ检验表明，符合3∶1的分离特点，说明 PSAG12-IPT基因以单基因形式在子代中稳定遗传。携带 PSAG12-IPT的F2株系平均叶绿素含量（55.4 mg·g-1）比不携带 PSAG12-IPT的F2株系（51.7 mg·g-1）提高7%，说明 PSAG12-IPT基因能够降低植株叶片叶绿素的降解速率，使叶片维持较长持绿时间，延缓叶片衰老。 |
| 英文摘要: |
| It is very important to develop wheat cultivars with high yield potential by inhibiting leaf senescence. In order to understand the application of PSAG12-IPT in wheat genetic improvement, the transgenic lines with PSAG12-IPT were crossed with Shaanmai 159, Zhoumai 18, Yuanfeng 175, Xinong 979 and Zhengmai 9023. The F2 progenies were divided into two groups, with and without PSAG12-IPT, determined by molecular markers closely linked to PSAG12-IPT. And the chlorophyll content were also evaluated for the above plants. The results showed that the separation ratio of plant with and without PSAG12-IPT in transgenic lines was 110∶40, which fitted the expected ratio 3∶1 (χ=0.213). The chlorophyll content of flag leaf in plants with and without PSAG12-IPT was 55.4 mg·g-1 and 51.7 mg·g-1, respectively, which indicated the former was higher than the latter,and PSAG12-IPT could keep plants stay-green for a relatively long stage by reducing chlorophyll degradation rate. |
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