| 姚俭昕,张传量，宋晓朋，许小宛，邢永锋，吕栋云，宋鹏博，杨孟于，孙道杰.基于90K芯片的小麦穗长和旗叶长QTL分析[J].麦类作物学报,2020,(11):1283 |
| 基于90K芯片的小麦穗长和旗叶长QTL分析 |
| Molecular Cytogenetic Identification of a New Wheat-Aegilops geniculata Roth Derived Germplasm 1003 with High Resistance to Powdery Mildew and Stripe Rust |
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| DOI：10.7606/j.issn.1009-1041.2020.11.01 |
| 中文关键词: 小麦 穗长 旗叶长 QTL |
| 英文关键词:Triticum aestivum L. Spike length Flag leaf length QTL |
| 基金项目:国家重点研发计划项目（2016YFD0101802）；陕西省重点研发计划项目（2018DXM-NY-010） |
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| 中文摘要: |
| 为进一步挖掘控制小麦穗长和旗叶长的QTL，以小偃81和西农1376构建的包含120个株系的F9∶10RIL群体为材料，于2016年10月至2017年6月和2017年10月至2018年6月分别在陕西杨凌和河南南阳（用2017YL、2017NY、2018YL和2018NY表示）进行试验，对4个环境下的穗长和旗叶长进行表型鉴定，并利用90K芯片构建的高密度遗传连锁图谱进行QTL定位。结果表明，所构建的遗传图谱覆盖了小麦21条染色体，图谱全长3 172.49 cM，平均图距0.57 cM。采用完备复合区间模型对4种环境下的表型值及BLUP育种值分别进行QTL定位，共检测到3个控制穗长的QTL，分布在2B、2D和5D染色体上；检测到2个控制旗叶长的QTL，均分布在5A染色体上，其中控制穗长的 Qsl.nwsuaf-2D、 Qsl.nwsuaf-5D和控制旗叶长的 Qfll.nwsuaf-5A.1能够在多环境下稳定表达，为主效QTL，表型变异解释率分别为18.34%～22.51%、9.57%~14.94%和9.48%～16.36%。该结果可为小麦MAS育种、NIL构建、候选区域筛选及图位克隆等提供参考依据。 |
| 英文摘要: |
| In order to further excavate the QTLs of wheat ear length and flag leaf length, the F9∶10 RIL population consisting of 120 lines constructed by Xiaoyan 81 and Xinong 1376 was used as material and were planted in Yangling, Shaanxi and Nanyang, Henan from October 2016 to June 2017 and from October 2017 to June 2018(represented as 2017YL, 2017NY, 2018YL and 2018NY,respectively).The phenotyping of spike length and flag leaf length under the four environments was carried out,the high-density genetic linkage map constructed by 90K SNP assay was used for QTL mapping. The results showed that the generated genetic map covers 21 wheat chromosomes; the linkage group is 3 172.49 cM, and the average map distance is 0.57 cM. A complete compound interval model was used for QTL mapping analysis with the phenotypic values and BLUP breeding values under the four environments.3 QTLs controlling spike length were detected on 2B, 2D and 5D chromosomes, and 2 QTLs controlling flag leaf length were detechted on 5A chromosome. Qsl.nwsuaf-2D,Qsl.nwsuaf-5D(controlling spike length) and Qfll.nwsuaf-5A.1(controlling flag leaf length) detechted multiple environments were major QTLs, explaining the phenotypic variation ranging from 18.34% to 22.51%, 9.57% to 14.94% and 9.48% to 16.36%,respectively.The results provide reference for wheat MAS breeding,NIL construction,candidate region screening and gene cloning. |
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