| 于利伟，陈爱艳，郭 雷，羊 阳，李慧敏，谢彦周，刘树伟，漆小泉，王中华，高 欣.小麦西昌69的EMS诱变系蛋白品质变异分析及种质筛选[J].麦类作物学报,2020,(2):154 |
| 小麦西昌69的EMS诱变系蛋白品质变异分析及种质筛选 |
| Study on Protein Quality Variations and Selection of Germplasm of EMS Mutation Lines from Wheat Xichang 69 |
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| DOI：10.7606/j.issn.1009-1041.2020.02.03 |
| 中文关键词: 小麦 品质 EMS 高分子量麦谷蛋白亚基 不溶性蛋白聚合体含量 |
| 英文关键词:Wheat Quality EMS HMW-GS Percentage of unextractable polymeric protein |
| 基金项目:陕西省重点研发农业一般项目（2018NY-030，2018NY-056）；大学生创新训练计划项目（201910712077） |
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| 中文摘要: |
| 为探讨甲基磺酸乙酯（ethyl methane sulfonate,EMS）诱变在小麦品质育种中的价值，了解EMS诱变小麦籽粒品质变异状况，筛选优良的变异材料，对1 667份西昌69的EMS诱变系M6代籽粒的蛋白质含量、淀粉含量、面筋含量、容重、面团形成时间和稳定时间等多个籽粒性状进行了测定，初步分析了诱变系的品质变化，并通过SDS-PAGE电泳法及高效液相色谱技术分析了其籽粒高分子量麦谷蛋白亚基（HMW-GS）的组分和不溶性蛋白聚合体的含量。共筛选到101份HMW-GS变异诱变系，分别为亚基缺失、亚基增生、亚基置换和亚基缺失且置换4种变异类型。其中，有28份诱变系的蛋白质含量、面筋含量、形成时间、稳定时间等多个籽粒品质性状及不溶性蛋白聚合体含量优于亲本，且千粒重相对稳定，可以作为优质种质资源用于小麦品质育种。 |
| 英文摘要: |
| In order to investigate the potential of mutagenesis method with ethyl methane sulfonate(EMS) in wheat quality breeding,to understand the variation of grain quality and to screen elite mutant materials,several grain traits:protein content,starch content,gluten content,test weight,dough development time and stability time of 1 667 M6 wheat mutation lines of Xichang 69 were determined,to analyze the variation of quality traits of the materials. The different compositions of high-molecular-weight glutenin subunits(HMW-GS) were determined by SDS-PAGE,and the content of the unextractable polymeric protein was analyzed by high performance liquid chromatography.A total of 101 HMW-GS mutant lines were screened out with four variation types:subunit deletion,subunit addition,subunit conversion and subunit deletion and conversion. Among them,28 mutants were selected with better quality trait and relatively stable thousand-kernel weight,compared to those of their parent,which canserve as valuable germplasm resources for wheat quality breeding in the future. |
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