| To provide useful information for wheat breeding in the south of Huang-Huai winter wheat region, 25 Australian wheat germplasm were planted in Xinxiang during 2016-2018, some agronomic traits of these germplasm were investigated, and protein parameters and high quality subunit of these germplasm were analyzed. The result showed that, many of Australian wheat germplasm expressed spring habit and poor integrated agronomic trait; the thousand kernel weight of Australian wheat germplasm, ranging from 31.4 g to 43.1 g, was lower than that of Zhoumai 18. The variation range of wet gluten content,gluten index, stability, and degree of softening were 28.0%-39.1%, 55.2%-96.5%, 4.0 min-25.0 min and 5%-100%, respectively. Wheat germplasm with high gluten content, high wet gluten index, long stability and white flour color were screened out, and wheat germplasm with high quality 5+10 subunits, 7OE subunits, 17+18 subunits, 13+16 subunits, low PPO activity gene and Wx-B1 gene missing, and wheat germplasm containing Rht-D1b dwarfing gene, Lr34 and Lr46 resistant leaf rust gene were selected from Australian wheat germplasm. A combination of three or four crosses was prepared with wheat varieties with outstanding performance in Huang-Huai winter wheat region, and Australian wheat germplasm as modified parent. New wheat lines with excellent integrated agronomic traits and outstanding quality were bred with good traits and directionally selected by conventional pedigree method.