| 吴 凡，徐德芳，宋少帅，李超卿，穆 平.小麦丙氨酸-乙醛酸转氨酶基因（ TaAGT2）的克隆、生物信息学预测及表达分析[J].麦类作物学报,2019,(8):895 |
| 小麦丙氨酸-乙醛酸转氨酶基因（ TaAGT2）的克隆、生物信息学预测及表达分析 |
| Cloning,Bioinformatics Prediction and Expression Analysis of Alanine-glyoxylate Aminotransferase Gene( TaAGT2) in Wheat(Triticum aestivum L.) |
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| DOI：10.7606/j.issn.1009-1041.2019.08.02 |
| 中文关键词: 小麦 AGT2 RACE 生物信息学 qRT-PCR |
| 英文关键词:Wheat AGT2 RACE Bioinformatics qRT-PCR |
| 基金项目:山东省现代农业产业体系小麦创新团队项目（SDAIT-01-05）；山东省农业良种工程项目( 2016LZGC-023)；青岛农业大学研究生创新项目（QYC201719） |
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| 中文摘要: |
| 光呼吸是植物细胞H2O2的重要来源，也是维持细胞氧化还原的重要成分，影响植物对生物和非生物逆境的应答，因此，挖掘与光呼吸有关的基因对提高植物的抗逆性具有重要意义。本研究以耐旱小麦品种青麦6号转录组数据为基础，通过RACE技术克隆得到小麦 AGT2基因的全长cDNA，将其命名为 TaAGT2，并对其进行了生物信息学预测及表达模式分析。结果表明， TaAGT2开放阅读框长1 434 bp，编码477个氨基酸。该蛋白属于亲水性稳定蛋白，定位于线粒体中，二级结构以α-螺旋（42.14%）和无规则卷曲 （32.91%）为主。 TaAGT2基因包含AAT- I基因族保守区域，与二穗短柄草 AGT2的相似性高达97%。通过qRT-PCR对基因 TaAGT2在不同组织中（根、茎和叶）及4种非生物胁迫（低温、ABA、干旱、高盐）下的表达特性进行分析，结果表明， TaAGT2在根、茎和叶中均有表达,茎中的表达显著高于根和叶，在不同胁迫条件下上调表达，表明该基因可能参与调控植物的抗逆反应。 |
| 英文摘要: |
| Photorespiration is an important source of H2O2 in plant cells and an important component in maintaining cell redox,which affects the response of plants to biotic and abiotic stresses.Therefore,it is important to excavate genes related to photorespiration to improve plant resistance. Based on the transcriptome data of drought-tolerant wheat variety Qingmai 6,the full-length cDNA of wheat AGT2 gene was cloned by RACE technology,named as TaAGT2,and its bioinformatics prediction and expression pattern analysis were carried out. The results showed that the open reading frame of TaAGT2 was 1 434 bp,encoding 477 amino acids. The protein is predicted to be a hydrophilic stable protein and localized in mitochondrion. The secondary structure is dominated by α-helix (42.14%) and irregular coiling (32.91%). The TaAGT2 gene contains a conserved region of the AAT-I gene family,which is as much as 97% similar to AGT2 of Brachypodium distachyon. The expression characteristics of the gene TaAGT2 in different tissues (roots,stems and leaves) and four abiotic stresses (low temperature,ABA,drought,and high salt) were analyzed by qRT-PCR. The results showed that the gene TaAGT2 expressed in roots,stems and leaves. The expression in stems was significantly higher than that in roots and leaves. It was up-regulated under different stress conditions,indicating that the gene may be involved in the regulation of plant stress resistance. |
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