| 李亚飞，阳文龙，顾晶晶，张爱民，詹克慧.小麦GRAS基因家族的全基因组鉴定与分析[J].麦类作物学报,2019,(5):549 |
| 小麦GRAS基因家族的全基因组鉴定与分析 |
| Genome-wide Identification and Characterization of the GRAS Gene Family in Bread Wheat(Triticum aestivum L.) |
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| DOI：10.7606/j.issn.1009-1041.2019.05.06 |
| 中文关键词: 小麦 GRAS基因家族 系统发育分析 表达分析 |
| 英文关键词:Triticum aestivum L. GRAS gene family Phylogenetic tree Expression analysis |
| 基金项目:国家重点基础研究发展计划(973)项目（2014CB138100）; 中国科学院战略性先导科技专项（XDA08010104） |
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| 中文摘要: |
| GRAS基因是一类转录因子基因，在植物的生长和发育中起关键作用。本研究利用最新的小麦基因组数据，对小麦GRAS基因进行了全基因组鉴定和分析。结果从小麦中鉴定出153个GRAS基因，这些基因不均匀分布在小麦21条染色体上。系统发育分析将这些基因分为12个亚家族，片段复制和串联重复是导致该基因家族扩张的主要原因。蛋白质序列分析发现不同亚家族间氨基酸数目、分子量存在一定差异；二级结构预测表明，小麦GRAS基因的氨基酸序列均以α-螺旋、随机卷曲为主要组成部分。通过对小麦GRAS基因在不同组织和不同逆境胁迫下的表达分析发现，GRAS基因在不同组织和逆境胁迫下存在明显的差异表达，表明GRAS基因具有组织或器官表达特异性，且可能在对逆境胁迫的响应中起重要作用。这些结果为进一步研究小麦GRAS基因的功能奠定了基础。 |
| 英文摘要: |
| The GRAS gene is a kind of transcription regulator family that plays a key role in plant growth and development. The present study identified and characterized the GRAS gene family in bread wheat using the latest wheat genome data. The results showed that 153 GRAS genes were identified and they are unevenly distributed on 21 chromosomes of wheat. Phylogenetic analysis showed that the GRAS genes can be divided into at least 12 subfamilies, and fragment duplication and tandem repeats are the main expansion ways of this gene family. Protein sequences analysis found that there are different number of amino acid residues and different molecular weight among different subfamilies, and their secondary structures mainly compose of α-helix and random coils. The expression analysis of wheat GRAS genes showed that they differentially expressed in different tissues and under different stresses, indicating that wheat GRAS genes specifically expressed in different tissues and responded to different stresses. These results lay a foundation for further study on the function of GRAS genes in wheat. |
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