To investigate the physiological response of wheat to post-anthesis high temperature,16 wheat cultivars of Huang-Huai-Hai region were used to measure the content of soluble protein,soluble sugar,malondialdehyde(MDA) and activity of superoxide dismutase(SOD) in flag leaf of wheat under high temperature during grain filling stage. The changes of grain weight and yield were analyzed and the differences of resistance to high temperature at the late grain filling stage were compared. The results showed that compared with the control,after high temperature treatment for seven days,soluble protein content,soluble sugar content,and SOD activity in wheat flag leaf were increased,while the content of MDA was decreased. After high temperature treatment for 15 days,soluble protein content,SOD activity and MDA content were increased compared with the control,while soluble sugar content was decreased. After high temperature treatment,grain weight and yield of all cultivars were decreased. Among them,grain weight and yield of Yumai 49-198 and Zhengmai 366 were decreased significantly. Through correlation analysis,principal component analysis,and subordinate function method and cluster analysis for comprehensive evaluation,we found that Jimai 22 and Shimai 19 showed greater heat resistance,and soluble protein content,soluble sugar content in flag leaf and 1 000-grain weight could be used as the heat tolerance indices. |