| In order to explore genes associated with kernel number per spike(KNPS) that stably expressed in different irrigation modes , the QTLs for KNPS were evaluated by the population of 302 recombinant inbred lines(RILs) derived from a cross between a drought-tolerant cultivar Luohan 2 and a water-sensitive cultivar Weimai 8 in three restrict watering and three irrigation environments. Totally 24 additive QTLs for KNPS were identified and distributed on chromosomes 2B, 3A, 3B, 3D, 4A, 4B, 5A, 5B, 6B and 7B, respectively, explaining 3.7% to 20.43% phenotypic variations. Of them, 14 additive QTLs located at 11 sites and 10 QTLs located at 6 sites in three irrigation environments and three drought stress environments, respectively. Among all the 16 sites detected, 9 sites were detected in the irrigation environment only, and 5 sites were detected in the dry farming environment only, and 2 sites were detected in the irrigation and dry farming environments simultaneously. Qknps-WL-3A, located between Xbarc012 and Xgpw2266 on 3A chromosome, was detected in E1, E4, E5, E6, simultaneously. Besides, it can explain more than 10% phenotypic variation in three environments and can be detected in all the three water stress environments. Therefore, it can be used as a candidate marker in molecular marker assisted breeding for new water-saving and high-yielding varieties. |
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