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作者	单位
王小婷，王文静，李波，杨雯晶，胡卫国	（1.河南省农业科学院小麦研究所/河南省小麦生物学重点实验室/农业部黄淮中部小麦生物学与遗传育种重点实验室/国家小麦工程实验室，河南郑州 450002；2.西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室，陕西杨凌 712100；3.陕西省农业机械鉴定推广总站，陕西西安 710003）

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中文摘要:

为了解小麦的 TaGB1基因特性、表达情况及其与双子叶植物同源基因的进化关系，以小麦品种济麦22为研究对象，采用同源克隆的方法获得小麦G蛋白β亚基编码区序列， TaGB1编码区全长1 143 bp，编码380个氨基酸，预测分子量为41 kD，基因组序列中包含6个外显子和5个内含子，分别位于小麦基因组的4A、4B、4D染色体上，不同拷贝的氨基酸同源性高达99.91%。 TaGB1基因结构中包含7个WD40保守域，表达产物位于胞质和质膜上。经系统发育进化关系分析，单子叶植物与双子叶植物的G蛋白β亚基分化形成两大分支； TaGB1在进化关系上与单子叶植物较近，而与拟南芥等双子叶植物较远。 TaGB1在ABA、盐、热和干旱胁迫条件下上调表达，植物的根、茎、叶等部位均有表达，叶片中表达量较高，说明该基因可能参与调控植物的抗逆反应。

英文摘要:

To understand the genetic characteristics and expression of TaGB1 in wheat and its evolutionary relationship with the homologous genes of dicotyledonous plants,we cloned TaGB1 encoding wheat G protein β subunit by using Jimai 22 as experimental material,and the coding region of TaGB1 is 1 143 bp in length,encoding 380 amino acids. The predicted molecular weight was 41 kD.The genome sequence contained six exons and five introns,which were located in the A,B,D chromosomes of the wheat genome,and the amino acid homology was up to 99.91%. The gene structure of TaGB1 contains seven-WD40 domains,and subcellular localization of TaGB1 was on plasma membrane and cytoplasm. Phylogenetic evolutionary relationship indicated that the G protein β subunit of monocotyledonous and dicotyledonous plants is divided into two large different branches and TaGB1 is close to monocotyledonous plants and alienated with dicotyledonous plants,such as Arabidopsis thaliana. TaGB1 was up-regulated under ABA,salt,high hot and drought stress,and expressed in roots,stems and leaves of plants. Higher expression level was detected in leaves,indicating that TaGB1 may be involved in the regulation of plant stress response.

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