| 刘宏魁，李世鹏，吴 颖，苏胜忠，单晓辉，李 贺，韩俊友，陈 孝，原亚萍.三个小麦-大麦2H二体异代换系的细胞学及补偿能力研究[J].麦类作物学报,2018,(6):649 |
| 三个小麦-大麦2H二体异代换系的细胞学及补偿能力研究 |
| Cytology and Compensatory Ability of Three Wheat-Barley 2H Disomic Substitution Lines |
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| DOI：10.7606/j.issn.1009-1041.2018.06.03 |
| 中文关键词: 小麦 大麦 代换系 补偿能力 |
| 英文关键词:Wheat Barley Substitution line Compensation ability |
| 基金项目:国家自然科学基金项目(31370352) |
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| 中文摘要: |
| 为了给小麦-大麦异代换系的进一步利用奠定基础，观察分析了三个小麦-大麦2H二体异代换系2H(A)、2H(B)和2H(D)花粉母细胞减数分裂中期的染色体构型。结果表明，三个代换系的染色体构型均为2n=21Ⅱ，含有一对大麦Betzes 2H染色体，在细胞学上是稳定的；三个代换系具有21Ⅱ的频率依次为92.56%、94.12% 和95.41%。同时，在花粉母细胞减数分裂后期观察到了落后染色体、染色体桥及不同步分裂等现象，其出现的频率为2H(A)大于2H(B)和2H(D)。从各代换系的生活力、不育率和其他农艺性状看，大麦2H染色体导入小麦背景后，对小麦2B、2D的补偿能力好于2A。 |
| 英文摘要: |
| The chromosomal configuration of three wheat-barley disomic alien substitution lines 2H（A),2H（B) and 2H（D) was observed and analysed. The frequencies of cells with 2n=21Ⅱ in pollen mother cells at meiotic metaphase I（PMCs MI ) for 2H（A),2H（B) and 2H（D) were 92.56%,94.12% and 95.41%,respectively. The GISH patterns showed that there was one pairing barley chromosome at PMCs MI. The results indicated that the substitution lines 2H（A),2H（B) and 2H（D) were stable. At the same time,the lagging chromosome,chromosome bridge and asynchronization division were observed in PMCs at meiotic anaphase,and the frequencies of 2H（A) was higher than that of 2H（B) and 2H（D). The barley chromosome 2H has good genetic compensation ability for the wheat chromosome 2B and 2D in vitality and sterility and other agronomic characters. |
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