| 杨 宁，包雨卓，赵浡彤，吕 岩，彭瞰看，田 宇，徐庆华，于 晶，王军虹，苍 晶.ABA及氟啶酮调控下冬小麦分蘖节抗寒相关蛋白的鉴定与分析[J].麦类作物学报,2018,(2):213 |
| ABA及氟啶酮调控下冬小麦分蘖节抗寒相关蛋白的鉴定与分析 |
| Identification and Analysis on Cold-Resistant Relative Proteins Regulated by ABA and Fluridone in Winter Wheat(Triticum aestivum L.) |
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| DOI：10.7606/j.issn.1009-1041.2018.02.13 |
| 中文关键词: 东农冬麦1号 双向电泳 ABA 氟啶酮 抗寒蛋白 |
| 英文关键词:Dongnongdongmai 1 Two dimensional electrophoresis ABA Fluridone Cold-resistant proteins |
| 基金项目:国家自然科学基金项目(31471423);国家自然科学人才培养基金科研训练项目(J1210069);黑龙江省自然科学基金项目(C201418) |
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| 中文摘要: |
| 为了研究ABA调控下冬小麦在蛋白质水平上的抗寒分子机制，以强抗寒冬小麦品种东农冬麦1号为材料，在外源ABA及氟啶酮处理后，于大田自然降温至4 ℃和-25 ℃时取分蘖节进行蛋白质双向电泳分析。对各处理组的双向电泳结果进行软件分析可知，4 ℃下得到可匹配的蛋白点587个，-25 ℃下得到可匹配蛋白点394个。筛选后选择差异显著的24个蛋白点进行质谱分析, 经鉴定这些蛋白包括逆境蛋白（WCI16、LTR-Rbps、CORs）、代谢相关蛋白（PGAM、PGK、26sRP、ATPase-β、GST1、GST、GSTF5）、转录翻译相关蛋白（BTFs、 eIF4A、eIF5A）和未知蛋白。 |
| 英文摘要: |
| In order to study the cold-resistant molecular mechanism in winter wheat which is on protein level and is regulated by ABA, the test material is dongnongdongmai 1 which is a strong cold-resistant wheat variety.After treated by exogenous ABA and Fluridone, tillering nodes in field were collected respectively at 4 ℃ and -25 ℃. A lot of differentially expressing proteins were analysed by two-dimensional gel electrophoresis analysis. The results showed that:there were 587 matching protein spots at -4 ℃ and 394 matching protein spots at -25 ℃; after screening from above, 24 protein spots were identified by protein mass spectrometry; differentially expressing proteins are stress proteins(WCI16, LTR-Rbps, CORs), metabolic proteins(PGAM, PGK, 26sRP, ATPase-β, GST1, GST, GSTF5), transcription and translation related proteins(BTFs, eIF4A, eIF5A) and unknown proteins. |
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